The new consensus sequence is written in nucleic acid notation, for which the most familiar characters are A, C, G, and T. These characters correspond to the DNA nucleotides adenine, cytosine, guanine, and thymine, respectively. Nucleic acid notation, however, also includes characters that can be used to spell out degenerate sequences, that is, sequences that retain their identity and function despite containing base locations that may be occupied by alternative bases.
Kadonaga and his team employed emerging genomic techniques and devised novel computational strategies to unlock the DNA sequence code for the human Inr. They also discovered that this sequence is located precisely at the start site of more than half of all human genes, underlining the importance of the human Inr in the human genome. Log in to leave a comment.
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Comments 0. Add comment Close comment form modal. I agree to the terms and conditions. You must accept the terms and conditions. These experiments are performed on a polyacrylamide gel under low ionic conditions. Steps of gel retardation assay Identify a promoter region of interest. Make a crude nuclear protein extract from the developmental stage that the gene of interest is being expressed.
Mix the promoter fragment of interest with the crude extract. Run the following samples: a. Phillip McClean.
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